【Detection principle】 ICHV, CPV and CDV antibodies in dog blood were quantitatively detected by fluorescence immunochromatography. Basic principle: there are T1、T2、T3 and C lines on the nitric acid fiber membrane. Combination mat is sprayed with fluorescent nano material markers that can specifically recognize three antibodies,the CPV / CDV / ICHV IgG antibody in the sample first combined with the nanomaterial markers forming complexes, and then chromatography upward, the complexs combined with the corresponding T1, T2 and T3 lines, when the excite light irradiate, nanometer material emission fluorescence signal, and the signal strength and ICHV, CPV and CDV antibody concentration in the samples into positive correlation.
【Detection purpose】 Infectious Canine Hepatitis virus (ICHV) belongs to adenovirus family, which can cause acute septic infectious diseases in dogs. Detecting the content of ICHV IgG antibody in dogs can reflect the immune situation of the body. Canine Parvovirus (CPV) belongs to the parvovirus genus of Parvoviridae, which can cause severe infectious diseases in dogs. Detecting the content of CPV IgG antibody in dogs can reflect the immune situation of the body. Canine Distemper Virus type I (CDV)belongs to the genus measles virus of paramucoviridae, which can cause severe infectious diseases in dogs. Detecting the content of CDV IgG antibody in dogs can reflect the immune situation of the body. Clinical significance: 1) for pre-immunization evaluation; 2) antibody titer detection after immunization; 3) early detection and diagnosis of ICHV/CPV/CDV infection.
