【Detection principle】 This product uses fluorescence immunochromatography to quantitatively detect the content of cProg in dog serum/plasma. Basic principle: T and C lines are marked on the nitrocellulose membrane, the T line is coated with cProg antigen a, and the binding pad is sprayed with a fluorescent nanomaterial labeled antibody b that can specifically recognize cProg. The cProg in the sample is first labeled with the nanomaterial. The antibody b binds to form a complex, and then chromatographs upward. The complex competes with T-line antigen a and cannot be captured; On the contrary, when there is no cProg in the sample, antibody b binds to antigen a. When the excitation light is irradiated, the nano material emits a fluorescent signal, and the strength of the signal is inversely proportional to the concentration of cProg in the sample.
【Test Purpose】 The concentration of Canine Progesterone (cProg) in serum is related to the estrus stage of the dog. Compared with LH, the concentration of cProg is always rising during the estrus period of the dog, which is easier to track and can reflect the estrus situation of the dog in real time. The best breeding time is 3-6 days after the peak of LH. The progesterone level corresponding to the best breeding time varies widely between different dogs, generally between 0-50ng/ml, but there are also some that exceed this range. Therefore, the degree of vaginal epithelial cell qualitative and dynamic real-time monitoring The combined assessment method of serum progesterone concentration can greatly increase the probability of pregnancy in dogs.
